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THE FEUTEX TOOLKIT

Single-Spike Standard Addition

Estimate original concentration from unspiked and spiked responses with dilution correction.

Laboratory workspace● Runs in your browser
01 Your input
02 Your result
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FormulaOriginal concentration = standard*spike*signal/(spiked*(volume+spike)-signal*volume); Volume dilution factor = (volume+spike)/volume

Worked example

Inputs

Original sample signal
2 signal units
Spiked sample signal
3 signal units
Original sample volume
10 mL
Added standard volume
1 mL
Standard concentration
13 concentration units

Expected results

Original concentration
2 concentration units
Volume dilution factor
1.1 ×

Load the example in the workspace to reproduce these results. Display rounding does not change the underlying calculation.

How to use this tool

  1. Estimate original concentration from unspiked and spiked responses with dilution correction.
  2. Set original sample signal, spiked sample signal, original sample volume, added standard volume, standard concentration in the indicated units.
  3. Calculate and compare original concentration, volume dilution factor.

Try this example

{
  "signal": 2,
  "spiked": 3,
  "volume": 10,
  "spike": 1,
  "standard": 13
}

Accuracy and scope

Single-spike linear response model with blank-corrected signals, volume additivity and identical matrix response.

Is my data uploaded?

This tool runs locally in your browser. Input and output are not sent to a processing server. Copy or download your result before leaving the page.